Terminator

Part:BBa_K4286903:Design

Designed by: Bingru Feng   Group: iGEM22_SZU-China   (2022-09-28)


cbh1 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 680
    Illegal AgeI site found at 24
    Illegal AgeI site found at 724
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 661
    Illegal BsaI.rc site found at 527
    Illegal BsaI.rc site found at 561
    Illegal SapI site found at 318


Design Notes

The Pcdh1-cdh1ls-mazEF-Tcdh1 circuit was integrated into pCAMBIA1302 vector by homologous recombination. We transformed the recombinant plasmid into Trichoderma atroviride by Agrobacterium-mediated transformation in order to achieve condition-triggered suicide of T. atroviride ultimately.


Source

The sequence of cbh1 terminator comes from genomic sequence of Trichoderma reesei.


References

[1]Zhong Y, Liu X, Xiao P, Wei S, Wang T. Expression and secretion of the human erythropoietin using an optimized cbh1 promoter and the native CBH I signal sequence in the industrial fungus Trichoderma reesei. Appl Biochem Biotechnol. 2011 Nov;165(5-6):1169-77. doi: 10.1007/s12010-011-9334-8. Epub 2011 Aug 16.